Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster fromAmycolatopsis mediterranei
نویسندگان
چکیده
منابع مشابه
Cloning, sequencing, and analysis of the griseusin polyketide synthase gene cluster from Streptomyces griseus.
A fragment of DNA was cloned from the Streptomyces griseus K-63 genome by using genes (act) for the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor as a probe. Sequencing of a 5.4-kb segment of the cloned DNA revealed a set of five gris open reading frames (ORFs), corresponding to the act PKS genes, in the following order: ORF1 for a ketosynthase, ORF2 for a chain length-deter...
متن کاملDirect evidence that the rifamycin polyketide synthase assembles polyketide chains processively.
The assembly of the polyketide backbone of rifamycin B on the type I rifamycin polyketide synthase (PKS), encoded by the rifA-rifE genes, is terminated by the product of the rifF gene, an amide synthase that releases the completed undecaketide as its macrocyclic lactam. Inactivation of rifF gives a rifamycin B nonproducing mutant that still accumulates a series of linear polyketides ranging fro...
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متن کاملGenetic analysis of polyketide synthase and peptide synthase genes of cyanobacteria as a mining tool for new pharmaceutical compounds
Cyanobacteria are considered a promising source for new pharmaceutical lead compounds and a large number of chemically diverse and bioactive metabolites have been obtained from cyanobacteria. Despite of several worldwide studies on prevalence of NRPSs and PKSs among the cyanobacteria, none of them included Iranian cyanobacteria of Kermanshah province. Therefore, the aim of this study was t...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1998.tb12861.x